Sequencing of cosmids

Ms F.J. Steers fsteers at crc.ac.uk
Wed Feb 10 07:45:42 EST 1993


Does anyone have any suggestions about sucessful sequencing of cosmids.  I have 
been trying to sequence cosmids using the "Sequenase 2.0" kit and the DMSO 
denaturing method and getting unreadable or no sequences.  I think that my 
technique is OK, as I,ve recently used the same protocol on some plasmids
and have been getting good results.

(please excuse my bad typing!)

Answers by email would be massively apreciated
Thanks

Fiona
HBGU, University College,
London.
fsteers at crc.ac.uk



More information about the Methods mailing list