Sequencing of cosmids
Ms F.J. Steers
fsteers at crc.ac.uk
Wed Feb 10 07:45:42 EST 1993
Does anyone have any suggestions about sucessful sequencing of cosmids. I have
been trying to sequence cosmids using the "Sequenase 2.0" kit and the DMSO
denaturing method and getting unreadable or no sequences. I think that my
technique is OK, as I,ve recently used the same protocol on some plasmids
and have been getting good results.
(please excuse my bad typing!)
Answers by email would be massively apreciated
Thanks
Fiona
HBGU, University College,
London.
fsteers at crc.ac.uk
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