Sequencing of cosmids

Ms F.J. Steers fsteers at
Wed Feb 10 07:45:42 EST 1993

Does anyone have any suggestions about sucessful sequencing of cosmids.  I have 
been trying to sequence cosmids using the "Sequenase 2.0" kit and the DMSO 
denaturing method and getting unreadable or no sequences.  I think that my 
technique is OK, as I,ve recently used the same protocol on some plasmids
and have been getting good results.

(please excuse my bad typing!)

Answers by email would be massively apreciated

HBGU, University College,
fsteers at

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