Depurination

RROHAN at UMAB.BITNET RROHAN at UMAB.BITNET
Thu Feb 11 11:31:00 EST 1993


.gf martin notebook a0

This may represent an oligo which is not completely deprotected.
Some time ago we observed a green fluorescence under UV illumination which
was slightly larger than the oligo band.  This was probably due to
guanine modification (JS Eadie and DS Davidson, Nuc Acids Res 1987, 15: 8333).
I thought that this problem had been cleared up with the newer synthesis
chemistries.
At any rate these oligos worked fine for PCR.

Rich Rohan
University of Maryland at Baltimore



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