protein concentrating/desalting

donald seto don at helix.nih.gov
Wed Feb 17 10:43:48 EST 1993


In article <9302171525.AA12166 at phobos.med.pitt.edu> bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:
>
>	I have used one small trick that might be of some help in reducing
>your loss in dialysis.  Instead of loading a dialysis tubing with the 
>sample, cut out a piece of the dialysis tubing, wash it as usual, then
>slit it so that you now have a dialysis sheet.  You can now float this
>dialysis membrane sheet on a petridish filled with the buffer you want
>it dialysed against.  
>	Now place about 50-100 ul of your sample as a drop on this floating
>piece and let it sit for about an hour or less.  You can carefully draw up
Millipore also sells a filter/membrane for this purpose..it's a little more
rigid than the dialysis membrane, and perhaps less susceptible to sinking!
--
don at helix.nih.gov
301-402-0580   Bldg10/Rm8D14    NIDDK/NIH       Bethesda, MD  20892



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