HELP RNA from PINE NEEDLES

RPSCOTT RSCOTT at IRRI.CGNET.COM
Wed Feb 17 08:30:57 EST 1993


From:	IN%"<@CGNET.COM:parkes at waikato.ac.nz>"  "parkes" 15-FEB-1993 12:18:15.57
To:	IN%"<@CGNET.COM:methods-and-reagents at net.bio.net>"  "methods-and-reagents"
CC:	
Subj:	HELP-RNA FROM A PINETREE??!

Hi. 

I'm a beginner to this molecular biology stuff and as my first project for my
Masters thesis I'm trying to produce a cDNA library from Pinus radiata needles.

Well I've been trying to isolate enough undegraded RNA to have a go at this and
have only gotten low yeilds.

Does ANYONE out there know a WONDER PROTOCOL or have any little bit of advice
that may help to increase the yeilds and give more regular UNDEGRADED yeilds.

ANY help would be appreciated.

Bryan Parkes

c/o Biology Secreatry
University of Waikato
Private Bag 3116
Hamilton  
New Zealand.  




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