Biotinylation of N-terminus only

Tae Shin tshin at husc8.harvard.edu
Wed Feb 17 22:16:55 EST 1993


I am interested in labelling a protein (not an antibody) using N-hydroxy-
succinimide biotin.  The standard protocol requires this labelling be done at
pH9, which should label all of the accesible lysine sidechains and the 
N-terminus.  I have heard that by lowering the pH (to about 6) one should be
able to label the N-terminus selectively.  Has anyone had any experience with
this?  Any comments would be welcome.  Thanks.

T.B.Shin
tshin at husc8.harvard.edu



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