quantDNA whenRNApresent

ann baker mouse at LAMAR.COLOSTATE.EDU
Fri Feb 19 14:33:40 EST 1993


My DNA prep results in RNA remaining with the DNA. I don't want to use
RNAse becasbecause I will lose some DNA going through phenol-chloroform-etoh
ppt. What alterntives might be used? I have access to Hoefer TK0 102 standard
kit, TKO 100 minifluorometer that is supposed to work with Hoechst dye and
be accurate for 0.1 ng/uL DNA - contaminants that are ignored include RNA
and protein etc. I don't know if this is bis benzimide dye or not; don't
know how accurate readings are.

AEM Baker
Bio Dept  CSU
Fort Collins 80523
303 491 5307
mouse at lamar.colostate.edu



More information about the Methods mailing list