5' phosphorylation reagent for oligos

Rod Bonfiglioli rbonfigl at waite.adelaide.edu.au
Mon Feb 22 02:00:30 EST 1993


hi there folks....
i am trying to make 5' phosphorylated synthetic oligos.  i have considered using
a kinase reaction to phosphorylate the oligos following synthesis, but in order
to get higher purity it seems better to actually make the oligos with a 5'
phosphorylation group alrady in place.  does anyone have experience of this and
maybe know a good source of reagents for such a procedure????
thanks in advance

rod
--
Rod Bonfiglioli, Waite Agricultural Research Institute,
University of Adelaide, South Australia.
e-mail rbonfigl at waite.adelaide.edu.au



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