5' phosphorylation reagent for oligos

Cheung C. Yue ccy at po.CWRU.Edu
Mon Feb 22 13:27:26 EST 1993


In a previous article, rbonfigl at waite.adelaide.edu.au (Rod Bonfiglioli) says:

>i am trying to make 5' phosphorylated synthetic oligos.  i have considered using
>a kinase reaction to phosphorylate the oligos following synthesis, but in order
>to get higher purity it seems better to actually make the oligos with a 5'
>phosphorylation group alrady in place.  does anyone have experience of this and
>maybe know a good source of reagents for such a procedure????
>

I think most DNA synthesizer reagent supplier (I know Cruachem does) sell
5'-phosphate on phosphoramidite which just adds the 5' phosphate.  I have 
never tried it so I don't know how well that works.

C Cho Yue
ccy at po.cwru.edu
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