Frowning bands on Southerns(?)

Nigel Walker nigel at pathos
Wed Feb 24 15:24:07 EST 1993

Howdy folks,

Has anyone else out there seen "frowning" bands on their Southerns?

Any idea what the problem might be?  The gels were (supposedly) run in 1
X TAE at around 55V.  The gels are 18 x 28 x 0.5cm and were run 4 to a
box.  Total buffer volume was probably 3L.

I've never seen this phenomenon before, but I usually only run two gels
per box.



      Nigel Walker              [>======<]
      Dept. of Plant Biology     \  Mu  /         (510) 642-7085  Lab
      351 Koshland hall           \    /          (510) 642-4995  Fax
      U.C. Berkeley                \  /           (510) 548-LEAF
      Berkeley, CA 94720            \/      nigel at

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