Good alternative for Quantitative PCR

Andre Hamel hamel at
Thu Feb 25 09:15:33 EST 1993

In article <9302221844.AA04759 at> bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:
>>     I am posting this question for a friend
>> who is not hard wired to the net yet.  Does anyone
>> know of a gene or genes (of house keeping variety) other than actin or
>> G3PD that can be used for standardizing quantitative PCR from
>> RTed mRNA extracted from mammalian tissue samples?  The problem
>> with these two genes, I gather, are the psuedo genes that amp
>> from the DNA also found the nucleic acid prep. There has been no luck 
>> so far with with DNAseI digestion, followed by phenol extraction and 
>> precipitation
>> (using glycogen as a carrier).  A simple fix is to use a house keeping gene
>> that has no psuedo genes.  Are there any currently used for this purpose?
>> Thanks in advance!!!!
>> ________________________________________
>> Dan Gietz
>> _________________________________________


Peter Watson and group in Shiu's lab down the hall from you guys appears
to have quant. RT PCR working nicely. Perhaps they may be of help to you.



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