Good alternative for Quantitative PCR

Andre Hamel hamel at ccu.umanitoba.ca
Thu Feb 25 09:15:33 EST 1993


In article <9302221844.AA04759 at phobos.med.pitt.edu> bsh at MED.PITT.EDU (Basavaraju Shankarappa) writes:
>>     I am posting this question for a friend
>> who is not hard wired to the net yet.  Does anyone
>> know of a gene or genes (of house keeping variety) other than actin or
>> G3PD that can be used for standardizing quantitative PCR from
>> RTed mRNA extracted from mammalian tissue samples?  The problem
>> with these two genes, I gather, are the psuedo genes that amp
>> from the DNA also found the nucleic acid prep. There has been no luck 
>> so far with with DNAseI digestion, followed by phenol extraction and 
>> precipitation
>> (using glycogen as a carrier).  A simple fix is to use a house keeping gene
>> that has no psuedo genes.  Are there any currently used for this purpose?
>> Thanks in advance!!!!
>> ________________________________________
>> Dan Gietz
>> GIETZ at BLDGHSC.LAN1.UMANITOBA.CA
>> _________________________________________


Dan

Peter Watson and group in Shiu's lab down the hall from you guys appears
to have quant. RT PCR working nicely. Perhaps they may be of help to you.

Cheers

Andre



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