Site directed mutagenesis oligo design

ww40 William_D_WARREN at UMAIL.UMD.EDU
Thu Feb 25 23:47:00 EST 1993


Is there anyone out there that could offer advice as to the
design of an oligonucleotide to introduce a 12bp deletion
via site directed in vitro mutagenesis.

Also I'd like to solicit opinions as to the easiest and most effective
method for doing a site directed in vitro mutagenesis.

William D. WARREN
Center for Agricultural Biotechnology     Email: ww40 at
University of Maryland at College Park    Phone: (301) 405-7681 


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