Frowning bands on Southerns(?)
hamel at ccu.umanitoba.ca
Fri Feb 26 18:15:27 EST 1993
In article <1mglh7$onv at agate.berkeley.edu> nigel at pathos.berkeley.edu writes:
>Has anyone else out there seen "frowning" bands on their Southerns?
>Any idea what the problem might be? The gels were (supposedly) run in 1
>X TAE at around 55V. The gels are 18 x 28 x 0.5cm and were run 4 to a
>box. Total buffer volume was probably 3L.
What was the current? 1x TAE with heavy gauge platinum wire electrodes at
55 V can give "high" current (greater than 100 mAmps).
Manitoba Vet. Virol.
Canada R3T 5S6
email: hamel at ccu.umanitoba.ca
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