Frowning bands on Southerns(?)

Andre Hamel hamel at ccu.umanitoba.ca
Fri Feb 26 18:15:27 EST 1993


In article <1mglh7$onv at agate.berkeley.edu> nigel at pathos.berkeley.edu writes:
>Howdy folks,
>
>Has anyone else out there seen "frowning" bands on their Southerns?
>
>Any idea what the problem might be?  The gels were (supposedly) run in 1
>X TAE at around 55V.  The gels are 18 x 28 x 0.5cm and were run 4 to a
>box.  Total buffer volume was probably 3L.

What was the current? 1x TAE with heavy gauge platinum wire electrodes at
55 V can give "high" current (greater than 100 mAmps). 

Andre Hamel
Manitoba Vet. Virol.
Winnipeg, Manitoba
Canada  R3T 5S6

email: hamel at ccu.umanitoba.ca



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