Mr. K.R. Raj
kraj at crc.ac.uk
Sat Feb 27 16:54:42 EST 1993
I would be gratefull if any of you out there can give me any information
about the MMTV LTR promoter. I have cloned the lac Z gene under the control
of this promoter and electroporated it into L cells (mouse cells) and
obtained magnificient blue cells when I stained the cells with X-gal solution.
The promoter was turned on even before addition of Dexamethasone. With tith
the addition of Dexamethasone, the intensity of the blue increased about
Now here is the problem: When I electroporated the same DNA (MMTV LTR-lac Z)
into human cervical keratinocytes under electroporation conditions that were determin
optimised for human cervical keratinocytes, I manage to get a good
number of cells to take up the DNA but the cells fail to express
the lac Z gene.
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