DNA purification

Ashok Aiyar axa12 at po.CWRU.Edu
Mon Jan 4 13:33:27 EST 1993


In a previous article, A8101DAL at vm.univie.ac.at (Martin Hetzer) says:

>Geneclean II glassmilk efficiently removes DNA 200 bases or longer from
>agarose gels. Who has tried to isolate shorter (50-200) fragments.

I routinely isolate 100 - 200 bp dsDNA from 2 % agarose gels.
I find that electroelution onto DEAE-cellulose paper (Whatman DE-81)
works well for me.

Ashok
-- 
  Ashok Aiyar   Department of Biochemistry, CWRU   axa12 at po.cwru.edu
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