Inosine in PCR primers

GWDGV1::SALBERT salbert at gwdgv1.dnet.gwdg.de
Fri Jan 8 08:58:50 EST 1993


Hi netters,
I have just designed set of primers including inosine instead of third
wobbling base in some codons. Unfortunately, they don't work. Sure, problem
can be elsewhere, but I would appreciate any comment and\or experiences
about use of inosine in designing oligonucleotide primer.

Thanks in advance,
			Stefan



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