Glycogen for DNA Precipitation

awalley at molbiol.ox.ac.uk awalley at molbiol.ox.ac.uk
Fri Jan 8 11:28:16 EST 1993


In article <ashok.203.726453182 at biochemistry.cwru.edu>, ashok at biochemistry.cwru.edu (Ashok Aiyar) writes:
> In article <9301072225.AA18226 at net.bio.net> KNECHT at UCONNVM.UCONN.EDU (Dave Knecht) writes:
> 
>>I remember reading somewhere of people using glycogen as a carrier for precipit
>>ation of DNA.  Can someone enlighten me as to a concentration to use, purity re
>>quired and any pluses or minuses of this procedure that you have encountered? J
>>Dave
> 
> I feel awful about saying this, but I just buy this stuff called Molecular 
> Biology Grade glycogen from Boehringer Mannheim.  I don't know the 
> concentration, but add 1 ul of it to precipitations of any volume.  It works, 
> and hasn't negatively interfered in any noticible may with any subsequent 
> steps such as restriction digestion, ligation, or in vitro transcription with 
> T7 RNA pol.

We also use it very successfully to maximise our yields with the three 
consecutive DNA precipitations required using the chemical mismatch technique.

A.J. Walley,
Institute of Molecular Medicine,
Oxford, England



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