Glycogen for DNA Precipitation

Stephanie Edelmann BIOSEE at UKCC.UKY.EDU
Fri Jan 8 16:33:11 EST 1993


In article <16B50CB07.BIOSEE at UKCC.UKY.EDU>
BIOSEE at UKCC.UKY.EDU (Stephanie Edelmann) writes:
 
>
>In article <9301072225.AA18226 at net.bio.net>
>KNECHT at UCONNVM.UCONN.EDU (Dave Knecht) writes:
>
>>
>>I remember reading somewhere of people using glycogen as a carrier for precipit
>>ation of DNA.  Can someone enlighten me as to a concentration to use, purity re
>>quired and any pluses or minuses of this procedure that you have encountered? J
>>Dave
>
>We also must be using the Glycogen from Boehringer Mannheim.  Add 1ml to
                                                                   ===
Oops, I goofed, that's supposed to be 1ul (microliter!!!!)
 
>the DNA in ethanol and spin.  Glycogen is supposed to be a volume excluder
>(as far as I know), but we in our lab are convinced, that its main
>effect is a psychological one.  After adding glycogen, you all a sudden
>see a pellet.  That might be mostly glycogen, but it's a PELLET as oppose
>to nothing visible.  We haven't noticed any negative effects, yet. I've
>used it on DNA that was supposed to be cut, ligated, electrophoresed and
>probably others.
>
>Stephanie



More information about the Methods mailing list