Glycogen for DNA Precipitation

Martin Kennedy cytogen at chmeds.ac.nz
Fri Jan 8 17:03:14 EST 1993


In article <9301072225.AA18226 at net.bio.net>, KNECHT at UCONNVM.UCONN.EDU (Dave Knecht) writes:
> I remember reading somewhere of people using glycogen as a carrier for precipit
> ation of DNA.  Can someone enlighten me as to a concentration to use, purity re
> quired and any pluses or minuses of this procedure that you have encountered? J
> Dave
-- 
I use glycogen routinely as a carrier, and add it to DNA precipitations just as
you would add tRNA or LPA.  I tend to add about 5-10ug when precipitating small
amounts of DNA with either isopropanol or ethanol.  It works fine, doesn't
interfere with anything as far as I can tell (sequencing, cloning,
transfections etc) and isn't visible on gels.  I use Boehringer Molecular
Biology grade (Cat no 901-393) diluted in water to 1ug/ul, stored at -20 and it
lasts years, through multiple freeze thaw cycles.


Cheers,

Martin

Martin A Kennedy (E-mail = cytogen at chmeds.ac.nz)
Cytogenetic and Molecular Oncology Unit
Christchurch School of Medicine
Christchurch, New Zealand		



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