Glycogen for DNA Precipitation

Darin O'Brien obrien at
Sat Jan 9 16:55:39 EST 1993

I routinely use 1/50 volume of 10mg/ml glycogen for precipitation of nucleic
acids.  I make up a stock in sterile water and just use the cheap stuff 
from Sigma (cat#G-8751).  I have heard of people extracting the stuff before
using it but I haven't found the need so I don't.  I precipitate on ice for 
10 minutes before centrifuging.  Best of luck.

(obrien at

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