Dispersion of Agarose

Tom Schneider toms at fcsparc6.ncifcrf.gov
Sun Jan 10 17:43:20 EST 1993


In article <01GTD30FXAKG0000FE at irri.cgnet.com> RSCOTT at IRRI.CGNET.COM writes:
>I can't help cursing myself whenever I prepare agarose gels and
>the suspension happens to overboil inside the microwave.

We had a pressure cooker attached to a timer.  Put the agaraose/buffer in, make
sure there is some water in the bottom, set the timer to 20 minutes, and go do
something else.  20 minutes later you have nice hot agar and it never boils
over!  No aggravation---but one does need to think ahead a bit.

  Tom Schneider
  National Cancer Institute
  Laboratory of Mathematical Biology
  Frederick, Maryland  21702-1201
  toms at ncifcrf.gov



More information about the Methods mailing list