Dispersion of Agarose

RSCOTT at IRRI.CGNET.COM RSCOTT at IRRI.CGNET.COM
Mon Jan 11 19:11:34 EST 1993


In article <01GTD30FXAKG0000FE at irri.cgnet.com>, RSCOTT at IRRI.CGNET.COM writes:
> I can't help cursing myself whenever I prepare agarose gels and
> the suspension happens to overboil inside the microwave. At
> certain equally irksome times (especially with bigger preparations)
> the gel remained undispersed while considerable volume has already
> been lost during heating. I always cover the boiling flask
> with a beaker but this didn't do so much to prevent excessive
> evaporation. I discovered a trick (perhaps I just rediscovered it on
> my own) to avoid these pitfalls. I tried presoaking the gel overnight
> at the eve of my gel run. Wholla! The agarose dispersed quicker in the
> buffer. Dispersion took only about half the normal boiling time. By the
> time vigorous boiling commences the agarose particles were substantially
> dissolved. Volume loss is therefore minimized. Presoaking allows
> the gel to swell and be hydrated. Alternatively, one can equilibrate
> the gel suspension in a 55-650C bath (ca. 30 min) prior to boiling.
> The same result is achieved. I think it's not much trouble having to
> prepare the suspension well in advance and stand it for a while
> especially if the gel consistency can be maintained for different
> preparations of equal concentration.
>
> R. P. Scott

I apologize for not mentioning that I do the overnight presoaking in the
ref in order to avoid microbial growth. Thanks Andrew Cockburn for raising
the issue of microbial contamination.

R.P. Scott
IRRI
E-mail: rscott at irri.cgnet.com



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