Purity of DNA (Ratio of OD = 1.8-2.0) ?
Michael CHENG
a52041 at sakura.kudpc.kyoto-u.ac.jp
Tue Jan 12 01:11:03 EST 1993
Hi! I need help with a question that one of the student asked me during lab
today:
Why is a preparation of DNA considered to be pure if OD260/OD280 => 1.8?
Actually, I've never thought of it myself so I went around asking my labmates.
Well, nobody really knows the answer. Then I heard that there's possibly a
theoretical formula that sets the above value range.
If this is true, I would appreciate it if someone out there can point me to
the reference.
Thanks in advance.
Michael CHENG
Dept. of Biological Chemistry
Faculty of Pharmaceutical Sciences
Kyoyo University
Japan
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