Purity of DNA (Ratio of OD = 1.8-2.0) ?

Michael CHENG a52041 at sakura.kudpc.kyoto-u.ac.jp
Tue Jan 12 01:11:03 EST 1993


  Hi! I need help with a question that one of the student asked me during lab
today:
  Why is a preparation of DNA considered to be pure if OD260/OD280 => 1.8?
  Actually, I've never thought of it myself so I went around asking my labmates.
Well, nobody really knows the answer. Then I heard that there's possibly a
theoretical formula that sets the above value range.
  If this is true, I would appreciate it if someone out there can point me to
the reference.
  Thanks in advance.

Michael CHENG
Dept. of Biological Chemistry
Faculty of Pharmaceutical Sciences
Kyoyo University
Japan



More information about the Methods mailing list