EMS mutagenesis

GARBRECHT fgarbrec at POST.ITS.MCW.EDU
Wed Jan 13 19:09:14 EST 1993


Does anyone have a protocol for inducing mutations in cell lines
using EMS.  I am interested in negatively selecting variants for
a number of surface molecules, but I don't know how much EMS to
use and under what specific conditions.
Thanks
Fred Garbrecht
Medical College of Wisconsin
fgarbrec at post.its.mcw.edu





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