reconstruction of whole genome

Basavaraju Shankarappa bsh at MED.PITT.EDU
Thu Jan 14 20:21:12 EST 1993


Hi Netfolks:
Recently there was a question about the feasibility of replicating
the whole genome in vitro (I think it was this group).
	Well I saw an article in Nucleic acids Research that might
be slightly relevant here.  A random-PCR method to construct whole
cDNA library from low amounts of RNA by Patrick Froussard.
NAR 20: 2900
	The steps are essentially using a universal primer containing a
random hexamer at the 3' end to make cDNA and amplifying with universal
primers.  The author shows that this process can make cDNAs as long as
4 kb starting from 10 x -6 ug of MS2 RNA.  I am not personally convinced
that this would be a true representation of the entire cDNA population
since he has not made any observations to substantiate the representation
of the genome.  The author claims that this method can provide for
construction of whole cDNA libraries from one eukaryotic cell, 100 bacteria
etc.,.
	Is it time to try reconstructing dinosaurus from the
blood of fossilized insects??? a la Michael Creighton (a great idea!).
Raj Shankarappa
bsh at med.pitt.edu




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