Deionization of old acrylamide

Bruce Roe broe at aardvark.ucs.uoknor.edu
Sun Jan 17 09:31:00 EST 1993


In article <2A507702 at coursmtp.nrc.ca>, pbonham at pbi.lan.nrc.ca writes...
>I don't know if you can re-deionise the acrylamide but why not just make less
>and store at 4oC in the dark.
> 
Good suggestion, also, why not try an experiment and compare three gels,
one made with old acrylamide, one made with old but Amberlite treated
acrylamide, and one made with fresh acrylamide, if you really want a
definative answer.  Then let us all know the results.  In the future
however, do store the concentrated gel mixes in the dark at 4oC as
recommended by the above note, since the components are photo-reactive.
Cheers......bruce
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