deleting one base

Fiberman wsun at jeeves.ucsd.edu
Tue Jan 19 18:38:33 EST 1993


This is a question for molbio experts:

I have a linear piece of double stranded DNA with the sequence
something like this:

5'- CGAAAA.....
3'- GCTTTT.....

I wish to delete the first base (C) so I would end up with:

5'- GAAAA....
3'- CTTTT....

what would be the best way to do this?  I need to end up with a
uniform population of DNA because I will be using it for a
ligation.

-fm



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