Colony Hybridisation with biotinylated oligo
Mr. A.F. Hill
ahill at crc.ac.uk
Wed Jan 20 07:45:58 EST 1993
I'm trying to set up a system for doing colony hybridisations using a
biotinylated oligo (23-mer) and using streptavidin-biotin-alkaline phosphatase
and NBT/BCIP to get the colour reaction...
most protocols i have read are for using probes labelled via nick-translation
or random priming and im wondering whether an oligo with one biotin group
will work the same...
ive spotted the oligo onto a filter and can read a signal down to 10pg but
when i hybe with the oligo and develop the colour i get a nice purple
can anyone help?
Andy Hill |
Prion Disease Group | E-Mail: ahill at uk.ac.crc
Dept. Biochemistry & Molecular Genetics |-----------------------------------
St Mary's Hospital Medical School | Phone: 071 723 1252 ext. 5469
LONDON W2 1PG | Fax: 071 706 3272
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