Cloning PCR Fragments

Bruce Elder elder at CGL.UCSF.EDU
Wed Jan 20 17:23:20 EST 1993


One other thing about cloning PCR fragments with T vectors just came to me.
If you use polymerases other than TaqPol (Vent, etc) you don't get as many
A overhangs on your PCR product. We have demonstrated this indirectly by
getting fewer clones from Vent amplified reactions. Everbody may already
know this, but I thought I'd throw it out there!

Bruce



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