insert-positive blue cols

EDWIN TEN DAM BEMDAM at rulgl.LeidenUniv.nl
Thu Jan 21 05:43:00 EST 1993


hi there,

i don't know about 500 bp, but i once insert 51 bp in a pUC vector. In com-
bination with the deletion resulting from cutting the polylinker, everything
was in frame. I checked quite some white colonies before i figured out what
was happening (8-() . I can tell you that weird things happen while cloning.
Out of the first 12 blue colonies, 11 were ok.

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             edwin ten dam - bemdam at rulgl.leidenuniv.nl



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