insert-positive blue cols
EDWIN TEN DAM
BEMDAM at rulgl.LeidenUniv.nl
Thu Jan 21 05:43:00 EST 1993
i don't know about 500 bp, but i once insert 51 bp in a pUC vector. In com-
bination with the deletion resulting from cutting the polylinker, everything
was in frame. I checked quite some white colonies before i figured out what
was happening (8-() . I can tell you that weird things happen while cloning.
Out of the first 12 blue colonies, 11 were ok.
edwin ten dam - bemdam at rulgl.leidenuniv.nl
More information about the Methods