SDS, nylon and hybridization

DMEIER at mis.mcw.edu DMEIER at mis.mcw.edu
Fri Jan 22 15:34:10 EST 1993


Hello to All!
  I am using Nytran nylon membranes in my Northern blotting. Often, I see 
SDS used in hybridizations, mostly with protocols using nylon filters. 
  Will anyone please share their expertise on this matter?  Is SDS
important in filter hybridizations, particularly with nylon?  Also, I've 
heard dextran works well with nitrocellulose, but that it is not 
recommended for nylon due to high background artifacts.
  Thanks much!

Dan
dmeier at mis.mcw.edu



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