CH3-ation of cytosine residues...how?

Vivian Miao vmiao at oregon.uoregon.edu
Thu Jan 21 23:38:38 EST 1993


In article <1j7ju0$73b at agate.berkeley.edu>, nigel at pathos (niggley mon)
wrote:

> Is there an easy way to methylate all the cytosine residues (5-m) of
> plasmid DNA in vitro?  If not, can I get 'coli to do it for me?

You can make 5 meC DNA by PCR (substitute 5meC in the 
nucleotide mix for C).  NEB also sells a CpG methylase that may suit your
purpose, if all the positions you are interested in are CpG dinucleotides.



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