CH3-ation of cytosine residues...how?
vmiao at oregon.uoregon.edu
Thu Jan 21 23:38:38 EST 1993
In article <1j7ju0$73b at agate.berkeley.edu>, nigel at pathos (niggley mon)
> Is there an easy way to methylate all the cytosine residues (5-m) of
> plasmid DNA in vitro? If not, can I get 'coli to do it for me?
You can make 5 meC DNA by PCR (substitute 5meC in the
nucleotide mix for C). NEB also sells a CpG methylase that may suit your
purpose, if all the positions you are interested in are CpG dinucleotides.
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