pGEX fusion vector expression

Fiberman wsun at jeeves.ucsd.edu
Fri Jan 22 19:39:20 EST 1993


I have just cloned my gene into the pGEX vector using XL1-blue
cells.  Now the question is this:  is XL1-blue a suitable cell
line for expressing my fusion protein?  If not, what is a good
cell line to use?  Anyone who have had experience with the pGEX
vector please respond.

-fm

ps.  pGEX is a glutathione S-transferase fusion vector.



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