Northern

Martin Kennedy cytogen at chmeds.ac.nz
Sun Jan 24 17:34:41 EST 1993


In article <9301231934.AA10759 at net.bio.net>, GWU5042 at GWUVM.GWU.EDU (Hans Xie) writes:

> Has anyone had exprience in mixing up two probes in one northern, such
> as one probe for detecting signal, another actin probe for normalization?
> How is it as compared to doing northern twice with stripping?
-- 

I don't think it would be wise to mix the actin probe with your test probe
unless you have an idea of the level of expression relative to actin.  I always
do my test probing first, then reprobe with actin without bothering to strip
off the first signal. 

Cheers,

Martin

Martin A Kennedy (E-mail = cytogen at chmeds.ac.nz)
Cytogenetic and Molecular Oncology Unit
Christchurch School of Medicine
Christchurch, New Zealand		



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