pGEX fusion vector expression

GWDGV1::SALBERT salbert at gwdgv1.dnet.gwdg.de
Sun Jan 24 12:21:11 EST 1993


-fm:
I have used pGEX cloning system and it worked just fine. In my procedure
E.coli DH5 alpha cells were used. But folks around me claims that XL-1
worked well for them.
The only possible drawback I assumed, that pGEX overproduces lac operator
(lac Iq gene product) itself. XL-1 strain overproduces the same protein
from its F'episome. So I was only affraid of over-over-production of lac Iq
gene product in XL-1 case (inhibition of cell growth, heterologous gene .
expression...??)
That's why I decided for DH5.

Hope it helps.
		Stefan



More information about the Methods mailing list