Shuttle vector- Hirt lysate yields

BMA14432 at VAX1.UTULSA.EDU BMA14432 at VAX1.UTULSA.EDU
Sun Jan 24 09:13:51 EST 1993


Britt,
I have found when performing the Hirt procedure it is very important
to GENTLY rock the tube(I do these in eppendorff tubes) after adding
the SDS. I have also found lysis to be improved if one warms the 
tube during this process(I hold it over a bunsenburner...not too close!:)).
I also use library competent cells(MC1061/P3 from Invitrogen). Hope that
Helps.

Mark Brudnak
University of Tulsa
BMA14432 at VAX1.UTULSA.EDU




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