browns at ccu.umanitoba.ca
Thu Jan 28 14:07:47 EST 1993
In article <9301251402.AA17477 at esds01.es.dupont.com> scolnipa at esvax.dnet.dupont.com writes:
>I'm running fairly long (52 cm) sequencing gels with labelled DNA
>fragments. Right now we have to cut the top 10 cm because we don't have
>X-ray film that's long enough. I recall seeing X-ray film in rolls
>somewhere. Does anybody recall brand, size and price?
>Also, we don't have a gel dryer that accomodates long gels. Any
>alternative drying method?
>Pablo A. Scolnik
I used to run really long gels in Dr. Yoder's lab a Cornell. Instead
of using a gel dryer, we would open the two glass plates (with the gel
sticking to the back plate-hopefully!), fix the gel on the plate in
Mehtanol/Acetic acid, and then dry the gel right onto the plate in a 95'C
oven. You can then put your X-ray film right on the plate, cover with
another glass plate (so it doesn't move around) and expose for however
long you need in a light tight box. If your are really intent on getting
the top of the gel, just cut another piece of X-ray film and put it on top.
After you are done with the gel, strip it off the glass by soaking overnight
in 3M NaOH.
Stuart M. Brown If you can remain cool when all
U. of Manitoba, Dept. Plant Science Around you are in panic,
Winnipeg, Manitoba, CANADA
browns at ccu.umanitoba.ca Then you surely misunderstand the situation
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