Problems: Retroviral Gene Transfer
bojar at uni-duesseldorf.de
Fri Jan 29 05:55:45 EST 1993
Does anyone have experience in generating high level retroviruses
for retroviral gene transfer? We want to use the PA317 retroviral packaging
line with the pLXSN retroviral vector. But we have some theoretical and
1.) Why produce a transfected helper line a lower virus titer
than a second helper line, which has been infected by a
transfected first? (esp. PA317 --> PE501)
2.) Does a transfected retroviral vector integrate into the
genome of the helper cell line? If not, how is the vector
replicated in a growing cell line under neo-selection?
( we could not detect an eucariotic oriR in pLXSN ).
3.) In order to generate a stable virus producing cell line, we need the
cell line PE501, the ecotropic derivat of PA317 (ATCC 9078), but we
could not find a soure for PE501.
****** Every answer helps
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