pGEX fusion vector expression

[Anthony Davies]

> I have just cloned my gene into the pGEX vector using XL1-blue
> cells.  Now the question is this:  is XL1-blue a suitable cell
> line for expressing my fusion protein?  If not, what is a good
> cell line to use?  Anyone who have had experience with the pGEX
> vector please respond.

Yes XL-1s support pGEX expression VERY well.

Anthony Davies
Institute of Virology
Oxford UK