Silver stain

[Bunka Sebastian]

Newsgroups: bionet.molbio.methds-reagnts
>Path: alijku05!alijku11!news.univie.ac.at!scsing.switch.ch!xlink.net!
 
>howland.reston.ans.net!darwin.sura.net!haven.umd.edu!uunet!psinnt
>p!iat.holonet.net!catsup
>From: catsup at iat.holonet.net (Mr. Christopher M. Gross)
>Subject: Silver Stain
>Message-ID: <C9EqJE.M0 at iat.holonet.net>
>Organization: HoloNet National Internet Access System: 
>510-704-1058/modem
>Date: Tue, 29 Jun 1993 23:57:12 GMT
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>Anyone -

>HELP!

>I have a silver stained gel in which the staining was to say the least
>overzealous and now the gel is all silver!  It is a rather important
>compound I am after and I need to destain and start again.

>Does anyone know how to get rid of the color?

>Respond here or catsup at holonet.net

>Thanks

>Christopher M. Gross

There is a very simple reaction adopted from photography (used
in many neutral silver staining protocols - and: IT WORKS!)

Make a dry mixture of:

Sodiumthiosulfate   30g
Sodiumbicarbonate 10g
Potassiumferricyanide  60g
-> store dry

use 1.5g in 300 ml aqua dest.

destain the gel 1-30 sec.; be careful: bands can disappear !

good luck,

+------------------------------------------------------------------+
|Dr. Sebastian Bunka      Email: Sebastian.Bunka at vu-wien.ac.at     |
|Inst.f.Bakteriologie                                              |
|Vet.Med.Uni Wien         Phone: +43-1-71155/261                   |
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|A-1030 Wien                                                       |
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