cleaning up PCR reactions

Ed Rybicki ED at micro.uct.ac.za
Fri Jul 2 02:40:28 EST 1993


> .....cleaning up PCR reactions.......
>
> I can demonstrate the removal of all detectable P32 end labeled primers from
> 500 bp PCR fragmnets by a simple single step isopropanol precipitation. Ed and
> I are using 1/5 vol of 10 M NH4Oac and 1 vol of isopopropanol. Spin
> immediately at room temperature for 20 minutes. If I am going to use this
> for sensitive (to NH4 ?) E. coli RNAP in vitro transcriptions, I pass them
> through a G-50 spin column as described in "the Maniatis". Another lab here
> uses templates prepared this way for direct sequencing with alternate primers.
>
> Why pay more ?
>
> Jim "kit-free" Graham

You mean you're using my "Kleen-It / Conc-It" Kit without ROYALTY
PAYMENTS???

  ____________________________________________________________________
 | Ed Rybicki, PhD             |       "Lord, won't you buy me        |
 | (ed at micro.uct.ac.za)        |                                      |
 | Dept Microbiology           |         A Mer-ce-des Benz..."        |
 | University of Cape Town     |                                      |
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