Double-Take(stratagene's mut. kit)?

Eugene Kim ekim at mail.sas.upenn.edu
Tue Jul 6 13:53:15 EST 1993


Has anyone tried or heard anything about Stratagene's new mutagenesis kit,
which claims 70% efficiency using DOUBLE-STRANDED plasmids! It should
theoretically speed up mutagenesis, especially if your using plasmids that
don't have m13 sequences for isolation of ss phage DNA. We're considering
giving it a try, but it's currently on back order. What I really wanted to
know was whether a) the efficiency is 70% as they claim, and whether large
plasmids have the same or similar efficiency of mutagenesis (the control
plasmid used was a bluescript plasmid with a 1.1 kb insert, according to
the hotline operator...). Any advice you can share with me about this would
be greatly appreciated.  Thanks!
Gene Kim
ekim at mail.sas.upenn.edu



More information about the Methods mailing list