Extracting DNA from low-melt agarose gels

KARN at Butler.EDU KARN at Butler.EDU
Thu Jul 8 07:03:12 EST 1993


I wish to use low-melt agarose gels to clean up a PCR product of about 1 kb
(+/- 0.2 kb) but would like to avoid the multiple extraction procedure 
described in Maniatis.  My goal is to sequence the product so it must be
relatively clean.  Anybody got an improvement on the Maniatis technique
or must I haul out the phenol?
Bob Karn
KARN at BUTLERU.edu



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