PCR on mouse spleen tissue
RICHARD P. PHIPPS
PHIP at BPHVAX.BIOPHYSICS.ROCHESTER.EDU
Thu Jul 8 13:42:55 EST 1993
Does anyone have any experience doing PCR or RT-PCR on mouse spleen?
We've been having trouble getting good amplification from RNA extracted
from spleen using the same methods we've had luck with for cell lines.
I know that doing PCR on blood requires special treatment, and I was
wondering if these might also be a good idea for spleen. I'd greatly
appreciate hearing about other people's experiences and strategies.
University of Rochester Cancer Center
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