Invitrogen PCR Optimizer: Comments?

RICHARD P. PHIPPS PHIP at BPHVAX.BIOPHYSICS.ROCHESTER.EDU
Fri Jul 9 11:47:25 EST 1993


In <1993Jul9.114827.3934 at ucbeh.san.uc.edu> engman at ucbeh.san.uc.edu writes:

> 
> I'm interested in trying the PCR Optimization kit ("Optimizer") from
> Invitrogen.  If anyone has any experience with this, I'd appreciate any
> comments (successes, failures, etc.) on the kit before I send away for it.
> 
It's certainly important to try as much as possible to optimize your PCR 
reactions for different primer sets.  I think the Invitrogen kit optimizes
on the basis of Mg++ concentration, pH, and the presence or absence of DMSO
in the reaction in a trial and error type system to see what combinations 
work the best.  

I would just copy the conditions used for the kit and try it yourself, instead
of paying however much for them to do it.

Rick Willis
University of Rochester Cancer Center



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