Plasmid Origins of Replication

Jennifer A. Thomson JAT at micro.uct.ac.za
Mon Jul 12 06:56:08 EST 1993


Hi netters
Does anyone know whether a pUC-based plasmid into which we clone an
origin of replication from pACYC will be stable and which ori will be
used in E. coli?  If the pACYC ori is cloned into an RK2-based plasmid
will this construct be stable and which ori will be used in E. coli?
Many thanks, Jennifer Thomson



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