Frequency of recombination

Dr Johnjoe Mcfadden bss1jm at surrey.ac.uk
Tue Jul 13 03:42:01 EST 1993


Does anyone know if recombination frequencies can be increased by
incresing the level of expression of eg. the recA gene in bacteria?

I am working with a bug that has a very low rate of recombination that
makes it difficult to do allele exchange etc.
Would it be possible to place the E.coli recA gene under the control of
a strong promoter, into these cells to increase the level of
recombination?

What happens if the E. coli recA gene is overexpressed?

Any ideas?

Thanks

Johnjoe McFadden
School of Biological Sciences
University of Surrey, Surrey, UK.
J.McFadden at UK.AC.SURREY



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