Site directed mutagenesis

Dr. S. Bhattacharya sbhattac at
Thu Jul 15 10:29:06 EST 1993

Hi netfolks
Could someone recommend a good reference on the principles of site
directed mutagenesis for the investigation of enzyme function.
We have no problems with the technique, but I would like to know
more about the general strategy, i.e. which mutants are likely to
provide the most information, what amino acid changes to make, and
where to make them.

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