Searching for CMV mammalian expression vector
hdang at cns.neusc.bcm.tmc.edu
Fri Jul 16 09:56:14 EST 1993
In article <btaylor.742800889 at muskwa.ucs.ualberta.ca>, btaylor at muskwa.ucs.ualberta.ca (Taylor Brian) writes:
|> hCMV IE1 promoter + intron A high level of expression
|> polylinker Xba, Not, BamH1, etc.
|> SV40 ori, polyoma ori
|> PolyA adenylation signal
|> pUC ori
|> Amp resistance marker
|> I have all of these components in one form or another, I'm just going
|> to have to do some PCRing, cutting and pasting to get them all together.
|> Does anyone know if this kind of vector has already been constructed,
|> and if so, how I might be able to get some? I don't mind taking a
|> little time out to make a vector, but perhaps its already been done.
|> Also, if anyone has any comments about "naked DNA" immunizations and
|> how they actually work, I'd like to hear from you.
|> Would adjuvants somehow help in eliciting a response?
|> Why do secreted proteins elicit a stronger response than membrane proteins?
|> And I have many, many more questions....
|> Thanks for your time,
|> Brian Taylor
|> Dept. of Medical Microbiology and Infectious Diseases
|> University of Alberta CANADA
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