Searching for CMV mammalian expression vector

Hong Dang hdang at cns.neusc.bcm.tmc.edu
Fri Jul 16 09:56:14 EST 1993


In article <btaylor.742800889 at muskwa.ucs.ualberta.ca>, btaylor at muskwa.ucs.ualberta.ca (Taylor Brian) writes:
|> 
|> hCMV IE1 promoter + intron A    high level of expression
|> polylinker  Xba, Not, BamH1, etc.
|> SV40 ori, polyoma ori
|> PolyA adenylation signal
|> pUC ori   
|> Amp resistance marker
|> 
|> I have all of these components in one form or another, I'm just going
|> to have to do some PCRing, cutting and pasting to get them all together.
|> 
|> Does anyone know if this kind of vector has already been constructed,
|> and if so, how I might be able to get some?  I don't mind taking a
|> little time out to make a vector, but perhaps its already been done.
|> 
|> Also, if anyone has any comments about "naked DNA" immunizations and
|> how they actually work, I'd like to hear from you.
|> Would adjuvants somehow help in eliciting a response?
|> Why do secreted proteins elicit a stronger response than membrane proteins?
|> 
|> And I have many, many more questions....
|> 
|> Thanks for your time,
|> 
|> Brian Taylor
|> Dept. of Medical Microbiology and Infectious Diseases
|> University of Alberta  CANADA
|> 	
|> 
 Invitrogen: pcDNA/Amp



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