Blunt end ligation debate.
drmax at casbah.acns.nwu.edu
Fri Jul 16 06:00:49 EST 1993
I gotta ask. How do you know that your CIAP step failed? Did you check your
cut ligase-minus vector for colony formation? Maybe you simply didn't get
all of the vector cut in the first place. Even with CIAPing I routinely 2x
gel purify my vector before using it for ligations and it is well worth the
effort. I rarely get more than one or two colonies on my control plate.
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