Agarose Gel Staining For Scanning/Digitizing...???
901106c at axe.acadiau.ca
Fri Jul 16 08:12:14 EST 1993
i just downloaded some nifty software from mac.archive.umich.edu called
"Gel Reader" which claims it will find lanes, bands, compare them to known
MW values, etc. Sounds great. One detail which I still am totally at a loss
for is: How can I stain an agarose gel to render it usefull for either
scanning or image capture with a ccd videocamera/frame grabber setup..?
Currently we use EtBr for all our gels, and for digitizing purposes, this
does not appear to do the trick. I was sent a procedure for Staining using
brilliant cresyl blue from Dr. Joseph Behnam at the university of reading,
but I've been having a great deal of trouble getting this procedure to work,
and I'm having trouble getting in touch with him.
So. If anyone can help me, or point me out to a reference that summarizes
proticols for agaros gel staining, I would greatly appreciate it!
Thanks very much!
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